槲皮素在大鼠上颌快速扩弓时对腭中缝骨改建的影响

doi:10.19439/j.sjos.2020.01.001

上海口腔医学 ›› 2020, Vol. 29 ›› Issue (1): 1-6.doi: 10.19439/j.sjos.2020.01.001

槲皮素在大鼠上颌快速扩弓时对腭中缝骨改建的影响

刘青^1,2, 陈菲³, 卢成焱⁴, 李静¹, 李晓乐¹, 张君¹

1.山东大学口腔医学院·口腔医院正畸科,山东省口腔组织再生重点实验室, 山东省口腔生物材料与组织再生工程实验室, 山东济南 250012;
2.泰安市妇幼保健院,山东泰安 271000;
3.日照市中医医院,山东日照 276800;
4.山东枣庄矿业集团中心医院,山东枣庄 277800

收稿日期:2018-12-24 出版日期:2020-02-25 发布日期:2020-03-09
通讯作者: 张君,E-mail: zhangj@sdu.edu.cn
作者简介:刘青（1984-）,女,硕士研究生,E-mail:645488628@qq.com
基金资助:
国家自然科学基金（81371180）; 山东省科学技术发展计划（2014GGH218024）

Effect of quercetin on bone formation in the mid-palatal suture of rats during rapid maxillary expansion

LIU Qing^1,2, CHEN Fei³, LU Chen-yan⁴, LI Jing¹, LI Xiao-le¹, ZHANG Jun¹

1.Department of Orthodontics, School and Hospital of Stomatology, Shandong University, Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration. Jinan 250012;
2.Taian Maternity and Child Care Hospital. Taian 271000;
3.Rizhao City Hospital Chinese Medicine.Rizhao 276800;
4.Central Hospital of Zaozhuang Mining Group of Shandong. Zaozhuang 277800, Shandong Province,China

Received:2018-12-24 Online:2020-02-25 Published:2020-03-09

摘要/Abstract

摘要： 目的：研究槲皮素对大鼠上颌快速扩弓时腭中缝骨质改建的作用。方法：18只6周龄SPF级雄性Wistar大鼠,随机分为3组,A组为空白对照组,B组为扩弓组,C组为扩弓并灌服槲皮素组,每组均6只。其中,A组不扩弓,也不灌服槲皮素;B﹑C组用双眼圈簧扩弓器扩弓,力值0.98 N,C组扩弓后每天灌服槲皮素（100 mg/kg）,A、B组根据体重灌服同剂量的生理盐水。14 d后处死大鼠,标本进行石蜡切片,行常规苏木精-伊红染色、Masson三色染色、免疫组织化学染色。利用Image-pro plus软件对切片吸光度进行分析,采用SPSS 19.0 软件包对数据进行统计学分析。结果：实验第14天,H-E染色和Masson染色显示,A组腭中缝见少量纤维组织,可见软骨细胞、间充质样细胞及成骨细胞等;B﹑C组腭中缝处纤维组织较多,成纤维细胞﹑软骨细胞增多,腭中缝近骨区域处多见成骨细胞;C组成骨细胞数量明显多于B组,有新骨钙化沉积。免疫组织化学染色显示,B组大鼠腭中缝组织中的BMP-2表达量在第14天时显著高于A组（P<0.01）,C 组大鼠腭中缝组织中BMP-2 的表达量显著高于B组（P<0.05）。结论：上颌快速扩弓可扩大生长发育期大鼠的腭中缝;槲皮素能够促进大鼠上颌快速扩弓时腭中缝BMP-2的表达,使骨质沉积钙化,加速新骨形成。

关键词: 槲皮素, 上颌快速扩弓, 骨改建, BMP-2

Abstract: PURPOSE: This study was aimed to investigate the effect of quercetin on rats' palatal suture during rapid maxillary expansion(RME). METHODS: Eighteen male 6-week-old（specific pathogen free,SPF） rats were randomly divided into 3 groups. Group A was control group, group B underwent expansion alone, and group C underwent expansion plus quercetin administration. Each group had 6 rats. Neither expansion nor quercetin was given to group A. Each rat in group B and C was set an orthodontic appliance with an initial expansive force (0.98 N). The rats in group C were administered with quercetin (100 mg/kg) at the same time every day after RME, while equal normal saline was given to rats in group A and B. Then the rats were sacrificed on day 14. Sections were cut perpendicular to the midpalatal suture and stained with hematoxylin and eosin (H-E) for observation of celluar response, and immunostained for evaluation of bone formation.The changes of collagen were observed through Masson's staining. Image-pro plus adhesive was used to analyze the slice absorbance, and SPSS19.0 software package was used to analyze the data. RESULTS: The results showed that small amount of fibrous tissue was found in the palate of group A, and there were chondrocytes, mesenchymal cells and osteoblasts. There were more fibrous tissues in the palate of group C, fibroblasts and cartilage cells increased, and osteoblasts were seen near the bone region of the palate. The number of osteoblasts in group C was significantly higher than that in group B, and there were new bone calcification deposits. The expression of BMP-2 in the midpalatal suture was significantly greater in group B than in group A at day 14 (P<; 0.01); the expression of BMP-2 in the midpalatal suture in group C was significantly greater than in group B (P<; 0.05). CONCLUSIONS: Rapid maxillary expansion can enlarge the middle palatal suture of rats during growth and development, quercetin can promote the expression of BMP-2 in the middle palatal suture of rats during rapid maxillary expansion, make bone deposition and calcification, and accelerate new bone formation.

Key words: Quercetin, Rapid maxillary expansion, Bone remodeling, BMP-2

中图分类号:

R783.5

刘青, 陈菲, 卢成焱, 李静, 李晓乐, 张君. 槲皮素在大鼠上颌快速扩弓时对腭中缝骨改建的影响[J]. 上海口腔医学, 2020, 29(1): 1-6.

LIU Qing, CHEN Fei, LU Chen-yan, LI Jing, LI Xiao-le, ZHANG Jun. Effect of quercetin on bone formation in the mid-palatal suture of rats during rapid maxillary expansion[J]. Shanghai Journal of Stomatology, 2020, 29(1): 1-6.

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槲皮素在大鼠上颌快速扩弓时对腭中缝骨改建的影响

Effect of quercetin on bone formation in the mid-palatal suture of rats during rapid maxillary expansion

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