mTOR及Tfr1 mRNA在腮腺黏液表皮样癌组织中的表达及与预后的关系

doi:10.19439/j.sjos.2019.05.010

上海口腔医学 ›› 2019, Vol. 28 ›› Issue (5): 499-503.doi: 10.19439/j.sjos.2019.05.010

mTOR及Tfr1 mRNA在腮腺黏液表皮样癌组织中的表达及与预后的关系

常攀辉¹, 艾克白尔·吐日洪², 李崴嵬³

1.首都医科大学附属北京口腔医院综合科,北京 100006;
2.和田地区人民医院口腔科,新疆和田 848000;
3.唐山市工人医院口腔科,河北唐山 063000

收稿日期:2019-01-16 出版日期:2019-10-25 发布日期:2019-12-11
通讯作者: 常攀辉,E-mail：jxbtwq@163.com
作者简介:常攀辉（1978-）,女,硕士,副主任医师
基金资助:
北京市科技新星计划（Z151100000315045）

Expression of mTOR mRNA and Tfr1 mRNA in mucoepidermoid carcinoma of parotid gland and their relationship with prognosis

CHANG Pan-hui¹, AKBEL Turgesjon², LI Wei-wei³

1.Department of General Dentistry,Beijing Stomatological Hospital affiliated to Capital Medical University.Beijing 100006;
2.Department of Stomatology,Hotan People's Hospital.Hotan 848000, Xinjiang Uygur Autonomous Region;
3.Department of Stomatology, Tangshan Workers' Hospital.Tangshan 063000, Hebei Province, China

Received:2019-01-16 Online:2019-10-25 Published:2019-12-11

摘要/Abstract

摘要： 目的探讨雷帕霉素靶蛋白（rapamycin target protein,mTOR） mRNA、转铁蛋白受体1（transferrin receptor 1,Tfr1） mRNA在腮腺黏液表皮样癌中的表达及与预后的关系。方法选取2013年6月—2015年7月唐山市工人医院口腔科收治的35例腮腺黏液表皮样癌患者为研究对象,检测腮腺组织mTOR及Tfr1 mRNA的相对表达量,分析mTOR及Tfr1 mRNA与腮腺黏液表皮样癌患者临床病理及预后的关系。采用SPSS 19.0 软件包对数据进行统计学处理。结果腮腺黏液表皮样癌组织mTOR及Tfr1 mRNA相对表达量显著高于癌旁组织（P<0.05）。mTOR及Tfr1 mRNA相对表达量与肿瘤浸润深度、肿瘤级别、临床分期及淋巴结转移相关（P<0.05）。预后良好组患者,mTOR及Tfr1 mRNA相对表达量显著低于预后不良组患者（P<0.05）。mTOR mRNA评估患者预后不良的AUC为0.815,高于Tfr1 mRNA的AUC（0.813）,两者联合检测评估患者预后不良的AUC为0.922,高于两者单独检测。Cox单因素及多因素分析显示,分化程度、TNM分期、mTOR mRNA、Tfr1 mRNA与腮腺黏液表皮样癌患者预后不良关系密切。结论腮腺黏液表皮样癌患者mTOR mRNA、Tfr1 mRNA相对表达量是否显著升高,可用于评估患者预后。

关键词: 雷帕霉素靶蛋白 mRNA, 转铁蛋白受体1 mRNA, 腮腺黏液表皮样癌, 预后, 相对表达量

Abstract: PURPOSE: To investigate the expression of rapamycin target protein (mTOR) mRNA and transferrin receptor 1(Tfr1) mRNA in mucoepidermoid carcinoma of parotid gland and its relationship with prognosis. METHODS: From June 2013 to July 2015 in Tangshan Workers' Hospital, 35 patients with mucoepidermoid carcinoma of parotid gland were selected. The relative expression of mTOR and Tfr1 mRNA in mucoepidermoid carcinoma of parotid gland was detected. The relationship between mTOR mRNA, Tfr1 mRNA and the clinicopathology and prognosis of mucoepidermoid carcinoma of parotid gland was analyzed using SPSS19.0 software package. RESULTS: The relative expression levels of mTOR mRNA and Tfr1 mRNA in mucoepidermoid carcinoma of parotid gland were significantly higher than those in adjacent tissues(P<0.05). The relative expression of mTOR mRNA and Tfr1 mRNA was correlated with invasion depth, tumor grade, TNM stage and cervical lymphatic metastasis(P<0.05). The relative expressions of mTOR and Tfr1 in patients with good prognosis were significantly lower than those in patients with poor prognosis (P<0.05). The AUC of mTOR mRNA was 0.815, higher than that of Tfr1 mRNA(0.813). The AUC of mTOR mRNA combined with Tfr1 mRNA was 0.922, higher than that of mTOR mRNA and Tfr1 mRNA alone. Cox univariate and multivariate analysis showed that differentiation, TNM stage, mTOR mRNA and Tfr1 mRNA were closely related to poor prognosis of mucoepidermoid carcinoma of parotid gland. CONCLUSIONS: The relative expression of mTOR mRNA and Tfr1 mRNA in patients with mucoepidermoid carcinoma of parotid gland was significantly increased, which can be used to evaluate the prognosis of patients.

Key words: Rapamycin target protein mRNA, Transferrin receptor 1 mRNA, Mucoepidermoid carcinoma of parotid gland, Prognosis, Relative expression

中图分类号:

R739.8

常攀辉, 艾克白尔·吐日洪, 李崴嵬. mTOR及Tfr1 mRNA在腮腺黏液表皮样癌组织中的表达及与预后的关系[J]. 上海口腔医学, 2019, 28(5): 499-503.

CHANG Pan-hui, AKBEL Turgesjon, LI Wei-wei. Expression of mTOR mRNA and Tfr1 mRNA in mucoepidermoid carcinoma of parotid gland and their relationship with prognosis[J]. Shanghai Journal of Stomatology, 2019, 28(5): 499-503.

参考文献

[1] 童国勇, 余周庆, 戈春城. 彩超引导下的粗针穿刺活检术在腮腺肿瘤诊断中的应用[J]. 口腔医学研究, 2017, 33(3):108-110.
[2] Rajasekaran K, Stubbs V, Chen J, et al.Mucoepidermoid carcinoma of the parotid gland: a national cancer database study[J]. Am J Otolaryngol, 2018, 39(3):321-326.
[3] Kissing S, Rudnik S, Damme M, et al.Disruption of the vacuolar-type H+-ATPase complex in liver causes MTORC1-independent accumulation of autophagic vacuoles and lysosomes[J]. Autophagy, 2017, 13(4):670-685.
[4] Li H, Choesang T, Bao W, et al.Decreasing TfR1 expression reverses anemia and hepcidin suppression in 2-thalassemic mice[J]. Blood, 2017, 129(11):1514-1526.
[5] Peng L, Song L, Liu C, et al.piR-55490, inhibits the growth of lung carcinoma by suppressing mTOR signaling[J]. Tumor Biol, 2016, 37(2):2749-2756.
[6] Magro G, Cataldo I, Amico P, et al.Aberrant expression of TfR1/CD71 in thyroid carcinomas identifies a novel potential diagnostic marker and therapeutic target[J]. Thyroid, 2011, 21(3):267-277.
[7] Vasudevan G, Bishnu A, Bmk S, et al. Mucoepidermoid carcinoma of salivary gland: limitations and pitfalls on FNA[J]. J Clin Diagn Res, 2017, 11(5):ER04-ER06.
[8] Sobral APV, Loducca SVL, Kowalski LP, et al.Immunohistochemical distinction of high-grade mucoepidermoid carcinoma and epidermoid carcinoma of the parotid region[J]. Oral Oncol, 2002, 38(5):437-440.
[9] Matthew EM, Hart LS, Astrinidis A, et al.The p53 target Plk2 interacts with TSC proteins impacting mTOR signaling, tumor growth, and chemosensitivity under hypoxic conditions[J]. Cell Cycle, 2009, 8(24):4168-4175.
[10] Kawabata H, Tong X, Kawanami T, et al.Analyses for binding of the transferrin family of proteins to the transferrin receptor 2[J]. Br J Haematol, 2015, 127(4):464-473.
[11] Smilevska T, Stamatopoulos K, Samara M, et al.Transferrin receptor-1 and 2 expression in chronic lymphocytic leukemia[J]. Leuk Res, 2006, 30(2):183-189.
[12] Nakamura Y, Nakamichi N, Takarada T, et al.Transferrin receptor-1 suppresses neurite outgrowth in neuroblastoma Neuro2A cells[J]. Neurochem Int, 2012, 60(5):448-457.
[13] 陈柯君, 程辉, 王健民, 等. mTOR通路下游核心分子的mRNA表达水平与初发AML患者临床特征的关系[J]. 中国临床医学, 2016, 23(1):17-21.
[14] Hicks MJ, El-Naggar AK, Flaitz CM, et al.Histocytologic grading of mucoepidermoid carcinoma of major salivary glands in prognosis and survival: a clinicopathologic and flow cytometric investigation[J]. Head Neck, 2010, 17(2):89-95.
[15] Shi L, Chen XM, Wang L, et al.Expression of caveolin-1 in mucoepidermoid carcinoma of the salivary glands: correlation with vascular endothelial growth factor, microvessel density, and clinical outcome[J]. Cancer, 2007, 109(8):1523-1531.

mTOR及Tfr1 mRNA在腮腺黏液表皮样癌组织中的表达及与预后的关系

Expression of mTOR mRNA and Tfr1 mRNA in mucoepidermoid carcinoma of parotid gland and their relationship with prognosis

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 6

编辑推荐

Metrics

本文评价

[1]	董淋升, 唐进, 程维, 冉婧璇, 刘兆博, 李雅冬. IL-6和β-catenin在口腔鳞癌中的表达及预后价值[J]. 上海口腔医学, 2023, 32(3): 280-286.
[2]	朱江, 许腾, 宋冬惠, 吴森斌. 口腔鳞状细胞癌组织中RACK1与M2/M1联合检测与预后的相关性分析[J]. 上海口腔医学, 2022, 31(5): 517-522.
[3]	叶璞, 黄兴鸿, 张伟, 吴萌, 俞辉明. cN0期舌鳞癌患者颈淋巴结转移的相关因素及预后分析[J]. 上海口腔医学, 2022, 31(5): 540-543.
[4]	王守鹏, 丁紫雪, 孟箭, 李梦, 李晓东, 袁壮. HIST1H2BH在头颈部鳞状细胞癌中的表达及临床意义[J]. 上海口腔医学, 2021, 30(6): 599-605.
[5]	贾舸, 于淼, 尚新华, 吴建华, 翟沁凯. 根管治疗慢性根尖周炎5年预后及影响因素分析[J]. 上海口腔医学, 2021, 30(2): 124-128.
[6]	丁红忠, 许从平, 周永敏. 晚期口腔鳞癌患者独立预后因素及生存预测模型的建立[J]. 上海口腔医学, 2019, 28(5): 536-540.