TC-PHBHHx/β-TCP复合物治疗SD大鼠干槽症的实验研究

doi:10.19439/j.sjos.2019.05.003

上海口腔医学 ›› 2019, Vol. 28 ›› Issue (5): 460-466.doi: 10.19439/j.sjos.2019.05.003

TC-PHBHHx/β-TCP复合物治疗SD大鼠干槽症的实验研究

孙小娟^1,2, 刘萍萍², 周忠伟^1,2, 秦立业², 付浩², 郑蓝天², 曹昆^1,2

1.宁夏医科大学总医院,宁夏银川 750004;
2.宁夏医科大学,宁夏银川 750004

收稿日期:2018-07-19 出版日期:2019-10-25 发布日期:2019-12-11
通讯作者: 曹昆,E-mail: ms_caokun@126.com
作者简介:孙小娟（1968-）,女,博士,主任医师、副教授,E-mail: ms_sunxiaojuan@126.com
基金资助:
2013年国家自然科学基金（81360173）; 宁夏教育厅教育改革项目（30200201）; 宁夏医科大学校级课题（XM2016054）; 宁夏自然科学基金（NZ17178）

Research of TC-PHBHHx/β-TCP compounds for treatment of dry socket in SD rats

SUN Xiao-juan^1,2, LIU Ping-ping², ZHOU Zhong-wei^1,2, QIN Li-ye², FU Hao², ZHENG Lan-tian², CAO Kun^1,2

1.General Hospital of Ningxia Medical University.Yinchuan 750004;
2.Ningxia Medical University.Yinchuan 750004, Ningxia Hui Autonomous Region, China

Received:2018-07-19 Online:2019-10-25 Published:2019-12-11

摘要/Abstract

摘要： 目的探讨不同制剂对干槽症的治疗作用,以期获得一种即抗菌又能加速成骨的治疗方法,临床应用提供理论依据。方法将72只SD雄性大鼠拔除左上前牙,在其拔牙窝形成干槽症。7 d后被随机分配到以下4组,接受不同治疗方法。A组—清创;B组—清创+碘仿纱条填塞;C组—清创+派丽奥软膏充填;D组—清创+TC-PHBHHx/β-TCP。治疗后的1、4、8周进行荧光标记,获得标本后,制作硬组织切片及脱钙切片,利用组织形态分析方法对各组成骨面积百分比进行比较。采用SPSS 19.0软件包对数据进行统计学分析。结果同一观察时间不同治疗组在成骨效果上相比, A、B、C 3组之间无统计学差异, 而D组与其他3组间存在统计学差异;相应地,可见有明显的新骨形成。同一治疗组不同观察时间在成骨效果上相比,B和C组间成骨面积无统计学差异, A组有少量骨形成,但伴随大量炎症细胞。然而,随着时间推移,D组表现出成骨面积逐渐增加的趋势。结论 TC-PHBHHx/β-TCP复合物具有显著的抗炎和成骨效果,碘仿纱条和派丽奥软膏具有良好的抗炎效果,但成骨效果不显著。

关键词: 干槽症, TC-PHBHHx/β, -TCP复合物, 骨再生, 大鼠

Abstract: PURPOSE: The effects of different compounds on dry socket were evaluated in order to find a new method that can both be antibacterial and osteogenic,providing experimental evidence for future clinical application. METHODS: Seventy-two male SD rats, with upper left anterior teeth been extracted, were infected by pus to result in dry socket.Seven days later, they were allocated randomly and evenly into 4 groups and received different treatment, i.e. group A: debridement; group B: debridement and filled with iodoform gauzes; group C: debridement and filled with periocline; group D: debridement and filled with TC-PHBHHx/β-TCP. After being treated for 1,4,8 weeks, sequential fluorescent labeling was performed. The animals were sacrificed after the procedure and hard tissue and decalcified sections were harvested for histological and histomorphometrical evaluation. Statistical analysis was performed using SPSS 19.0 software package. RESULTS: At the same time point, the results of osteogenesis in group A, B and C were not significantly different while the results in group D was significantly different from other groups; accordingly, significant new bone formation was observed. At different observation time, the bone area in group B and C were not significant different over time. In group A, little new bone formation was found but surrounded by a large amount of inflammatory cells at 8 week. However, group D showed bone area increasing gradually with time. CONCLUSIONS: TC-PHBHHx/β-TCP has significant anti-inflammatory and osteogenic effects. Iodoform gauzes and periocline have good anti-inflammatory results but not significant osteogenic effects.

Key words: Dry socket, TC-PHBHHx/β-TCP;, Osteogenesis, Rat

中图分类号:

R782.1

孙小娟, 刘萍萍, 周忠伟, 秦立业, 付浩, 郑蓝天, 曹昆. TC-PHBHHx/β-TCP复合物治疗SD大鼠干槽症的实验研究[J]. 上海口腔医学, 2019, 28(5): 460-466.

SUN Xiao-juan, LIU Ping-ping, ZHOU Zhong-wei, QIN Li-ye, FU Hao, ZHENG Lan-tian, CAO Kun. Research of TC-PHBHHx/β-TCP compounds for treatment of dry socket in SD rats[J]. Shanghai Journal of Stomatology, 2019, 28(5): 460-466.

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TC-PHBHHx/β-TCP复合物治疗SD大鼠干槽症的实验研究

Research of TC-PHBHHx/β-TCP compounds for treatment of dry socket in SD rats

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