姜黄素对去势骨质疏松大鼠下颌骨和股骨EZH2基因表达的影响

doi:10.19439/j.sjos.2019.03.004

上海口腔医学 ›› 2019, Vol. 28 ›› Issue (3): 241-245.doi: 10.19439/j.sjos.2019.03.004

姜黄素对去势骨质疏松大鼠下颌骨和股骨EZH2基因表达的影响

蒋琪, 朱冰玉, 德维, 周雄文

中南大学湘雅医院口腔科,湖南长沙 410008

收稿日期:2018-09-03 修回日期:2018-10-30 出版日期:2019-06-25 发布日期:2019-08-09
通讯作者: 周雄文,E-mail:zhouxw1128@163.com
作者简介:蒋琪（1992-）,男,硕士研究生,E-mail:408346700@qq.com
基金资助:
湖南省发改委科研项目[(2014)658号]

Effects of curcumin on EZH2 mRNA expression in the mandible and femur of ovariectomized osteoporosis rats JIANG Qi, ZHU Bing-yu, DEWI Chrystal, ZHOU Xiong-wen.

JIANG Qi, ZHU Bing-yu, DEWI Chrystal, ZHOU Xiong-wen

Department of Stomatology, Xiangya Hospital, Central South University. Changsha410008, Hunan Province, China

Received:2018-09-03 Revised:2018-10-30 Online:2019-06-25 Published:2019-08-09

摘要/Abstract

摘要： 目的研究姜黄素对去势骨质疏松大鼠下颌骨和股骨EZH2（enhancer of zeste homolog 2,EZH2）表达的影响,探讨其对骨质疏松大鼠的保护作用及机制。方法将30只6月龄SD雌性大鼠随机分为假手术组（SHAM组）、去势模型组（OVX组）和姜黄素治疗组(OVX+C组)。治疗组将姜黄素溶于羧甲基纤维素钠溶液后,按110 mg/kg·d灌胃给药;假手术组和去势模型组灌胃同等剂量羧甲基纤维素钠溶液,每天1次,连续12周后取材。检测大鼠血清钙、磷、碱性磷酸酶的指标变化,采用Micro-CT对左侧下颌骨和股骨的骨形态计量学参数进行测定。取右侧下颌骨和股骨,采用PT-PCR法检测骨组织EZH2mRNA的表达。采用SPSS22.0软件包对数据进行统计学分析。结果 OVX组骨组织中EZH2mRNA的表达显著高于SHAM组（P<0.05）。与OVX组相比,姜黄素可以改善骨质疏松大鼠的骨微结构,增加骨密度,降低骨质疏松大鼠血清碱性磷酸酶含量,使骨组织EZH2mRNA表达显著下调（P<0.05）。结论姜黄素能够防治去势大鼠的骨量丢失和改善骨微结构,其机制可能与下调EZH2mRNA的表达水平有关。

关键词: 去势大鼠, 姜黄素, 下颌骨, 股骨, 骨质疏松, EZH2基因

Abstract: PURPOSE: To study the effects of curcumin on EZH2 mRNA expression in the mandible and femur of ovariectomized osteoporosis rats,and to investigate its protective effect and mechanism. Methods: Thirty female 6-month old SD rats were randomly divided into sham group,OVX group and experimental group. The rats in the experimental groups were given curcumin (110 mg/kg) by intragastric administration after ovariectomy, while rats in the sham group and OVX group were given the same dosage of carboxymethylcellulose sodium solution, once a day for 12 weeks. All rats were sacrificed after the last intragastric administration. The serum samples were collected for detemination of biochemistrical parameters. Micro-CT was used for bone parameters and the morphology of the trabecular bone of the left mandibles and femurs. The expression level of EZH2mRNA in right mandible and femurs tissue was detected by reverse transcription polymerase chain reaction (RT-PCR). SPSS22.0 software package was used for statistical analysis. Results: The expression of EZH2mRNA in the OVX group was significantly higher than the sham group (P< 0.05). Compared with the OVX group,curcumin increased BMD and improved bone microstructure, decreased serum contents of alkaline phosphatase,and down-regulated the expression levels of EZH2mRNA in bone tissues of rats with osteoporosis (P< 0.05). Conclusions: Curcumin can effectively prevent the lose of bone volume of ovariectomized rats, and repaire bone microstructure. Its mechanism is related with down -regulation of EZH2mRNA.

Key words: Variectomized rats, Curcumin, Mandible, Femur, Osteoporosis, EZH2

中图分类号:

R780.2

蒋琪, 朱冰玉, 德维, 周雄文. 姜黄素对去势骨质疏松大鼠下颌骨和股骨EZH2基因表达的影响[J]. 上海口腔医学, 2019, 28(3): 241-245.

JIANG Qi, ZHU Bing-yu, DEWI Chrystal, ZHOU Xiong-wen. Effects of curcumin on EZH2 mRNA expression in the mandible and femur of ovariectomized osteoporosis rats JIANG Qi, ZHU Bing-yu, DEWI Chrystal, ZHOU Xiong-wen.[J]. Shanghai Journal of Stomatology, 2019, 28(3): 241-245.

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姜黄素对去势骨质疏松大鼠下颌骨和股骨EZH2基因表达的影响

Effects of curcumin on EZH2 mRNA expression in the mandible and femur of ovariectomized osteoporosis rats JIANG Qi, ZHU Bing-yu, DEWI Chrystal, ZHOU Xiong-wen.

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