白藜芦醇通过SOCS-1蛋白抑制牙髓卟啉单胞菌脂多糖诱导的氧化应激反应

doi:10.19439/j.sjos.2019.03.003

上海口腔医学 ›› 2019, Vol. 28 ›› Issue (3): 237-240.doi: 10.19439/j.sjos.2019.03.003

白藜芦醇通过SOCS-1蛋白抑制牙髓卟啉单胞菌脂多糖诱导的氧化应激反应

米靖^1,2, 于博^1,2, 于雅琼^1,2, 李晓琳^1,2, 杜亚鑫^1,2, 仇丽鸿^1,2

1.中国医科大学口腔医学院牙体牙髓病科,辽宁沈阳 110002;
2.辽宁省口腔医学研究所牙体牙髓病学研究室,辽宁沈阳 110002

收稿日期:2018-07-31 修回日期:2018-11-02 出版日期:2019-06-25 发布日期:2019-08-09
通讯作者: 仇丽鸿,E-mail:drqlh@yahoo.com
作者简介:米靖（1993-）,女,硕士研究生,医师,E-mail:540917148@qq.com
基金资助:
辽宁省高等学校科研基金（LFWK201718）

Resveratrol counteracts lipopolisaccharide-mediated oxidative stress by upregulating SOCS-1 production

MI Jing^1,2, YU Bo^1,2, YU Ya-qiong^1,2, LI Xiao-lin^1,2, DU Ya-xin^1,2, QIU Li-hong^1,2

1.Department of Endodontics, School of Stomatology, China Medical University. Shenyang 110002;
2.Lab of Endodontic, Liaoning Institute of Dental Research. Shenyang 110002, Liaoning Province, China

Received:2018-07-31 Revised:2018-11-02 Online:2019-06-25 Published:2019-08-09

摘要/Abstract

摘要： 目的探讨白藜芦醇（resveratrol,Res）对小鼠成骨细胞MC3T3-E1氧化应激（oxidative stress,OS）损伤的保护作用及作用机制。方法 RES及LPS刺激细胞后,荧光显微镜及流式细胞术检测细胞内活性氧水平;Western 免疫印迹法检测细胞内细胞因子信号转导抑制因子（suppressor of cytokine signaling-1,SOCS-1）蛋白表达水平。建立SOCS-1瞬时转染细胞系,利用荧光显微镜及流式细胞术分别观察转染后的细胞内活性氧水平。采用SPSS22.0软件包对数据进行统计学分析。结果 LPS组与空白组相比,细胞内氧化应激程度显著提高,LPS+RES组氧化应激程度显著低于LPS组（P<0.05）。10 mg/L P.e-LPS刺激30 min后,细胞内SOCS-1开始升高。SOCS-1转染后,LPS+RES组细胞内氧化应激程度显著高于未转染细胞内氧化应激水平（P<0.05）。结论白藜芦醇通过调节SOCS-1蛋白,减轻LPS引起的小鼠成骨细胞氧化应激水平,从而发挥保护作用。

关键词: 白藜芦醇, 氧化应激, SOCS-1, P.e-LPS

Abstract: PURPOSE: To investigate the protective effect and mechanism of resveratrol on oxidative stress of MC3T3-E1. Methods: The levels of reactive oxygen species in the cells were observed by fluorescence microscope and flow cytometry. The expression of SOCS-1 protein was detected by Western blot. SOCS-1 transient transfected cell line was established, and the levels of reactive oxygen species in transfected cells were observed by fluorescence microscopy and flow cytometry. The data were analyzed using SPSS22.0 software package. Results: The level of ROS in LPS group was significantly higher than that in the blank group and LPS+RES group (P<0.05). The expression level of SOCS-1 protein was increased after LPS stimulation for 30 min (P<0.05). The level of ROS in the siSOCS-1+LPS+RES group was significantly higher than that in the untransfected group (P<0.05). Conclusions: Resveratrol may counteract LPS-mediated oxidative stress in MC3T3E1 cells by modulating SOCS-1 protein.

Key words: Resveratrol, Oxidative stress, SOCS-1, P.e-LPS

中图分类号:

R781.3

米靖, 于博, 于雅琼, 李晓琳, 杜亚鑫, 仇丽鸿. 白藜芦醇通过SOCS-1蛋白抑制牙髓卟啉单胞菌脂多糖诱导的氧化应激反应[J]. 上海口腔医学, 2019, 28(3): 237-240.

MI Jing, YU Bo, YU Ya-qiong, LI Xiao-lin, DU Ya-xin, QIU Li-hong. Resveratrol counteracts lipopolisaccharide-mediated oxidative stress by upregulating SOCS-1 production[J]. Shanghai Journal of Stomatology, 2019, 28(3): 237-240.

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白藜芦醇通过SOCS-1蛋白抑制牙髓卟啉单胞菌脂多糖诱导的氧化应激反应

Resveratrol counteracts lipopolisaccharide-mediated oxidative stress by upregulating SOCS-1 production

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