上海口腔医学

上海口腔医学 ›› 2019, Vol. 28 ›› Issue (2): 123-127.doi: 10.19439/j.sjos.2019.02.003

• 论著 • 上一篇    下一篇

含锶交联藻酸钠凝胶对前成骨细胞黏附和增殖的影响

张漫1, 李昊1, 徐婷2, 刘杰1, *, 谈飞1, *   

  1. 1.青岛大学附属医院 口腔修复科,
    2.医保审核科,山东 青岛 266003
  • 收稿日期:2018-10-25 修回日期:2019-01-14 出版日期:2019-04-25 发布日期:2019-06-20
  • 通讯作者: 刘杰,E-mail:18661801995@163.com;谈飞,E-mail：Tanf-1984@163.com。*共同通信作者
  • 作者简介:张漫 （1992-）,女,硕士,E-mail：497205553@qq.com
  • 基金资助:
    山东省自然科学基金（ZR2018BH015）

Effect of strontium-containing cross-linked sodium alginate gel on adhesion and proliferation of pre-osteoblasts

ZHANG Man1, LI Hao1, XU Ting2, LIU Jie1, TAN Fei1   

  1. 1.Department of Prosthodontics, 
    2.Department of Medical Insurance,Affiliated Hospital of Qingdao University. Qingdao 266003, Shandong Province, China
  • Received:2018-10-25 Revised:2019-01-14 Online:2019-04-25 Published:2019-06-20

摘要： 目的 利用SrCl2作为交联剂,合成含锶交联新型藻酸凝胶,检测含锶藻酸凝胶的理化性质,并观察细胞于凝胶中的黏附、增殖情况。方法 按钙离子和锶离子比例,分别制备5组终浓度为4 g/L的电解液,分别与藻酸钠溶液交联。检测各组凝胶的压缩模量、溶胀率及锶离子析出浓度。将种子细胞MC3T3-E1接种于凝胶表面后,观察并行 FDA/PI 细胞染色。采用GraphPad Prism 6.0软件包对数据进行统计学分析。结果 藻酸盐凝胶在双蒸水及PBS中,溶胀率随Sr2+浓度增高而增大;压缩模量检测发现,C组凝胶强度显著高于其他各组。ICP显示,各组溶液的锶离子浓度逐渐降低。扫描电镜观察发现,凝胶孔隙随Sr2+浓度增加而增大。显微镜及FDA/PI染色均发现,种子细胞在C组凝胶表面的增殖明显改善。结论 当钙离子和锶离子等比例混合时,藻酸盐凝胶的细胞黏附和增殖性能明显改善,可作为一种新型可注射骨组织工程支架。

关键词: 藻酸盐凝胶, 锶, 细胞黏附, 细胞增殖, 组织工程

Abstract: PURPOSE: To study the physicochemical properties of bismuth-containing cross-linked alginate gel, to observe and detect the adhesion and proliferation of mouse MC3T3-E1 cells in gluten-containing cross-linked alginate gel and provides a theoretical basis for alginate injectable tissue engineering scaffolds. METHODS: Five sets of electrolytes with a final concentration of 4 g/L were prepared and cross-linked with sodium alginate solution, respectively. The compression modulus and swelling ratio of each group of gels were measured. Cells were inoculated on the surface of the composite gel, FDA/PI cells were stained to observe the adhesion morphology of the cells. Data were statistically analyzed using GraphPad Prism 6.0 software package. RESULTS: The swelling rate of double distilled water and PBS increased with the increase of Sr2+ concentration. The results of compression modulus showed that the strength of group C was significantly higher(P<0.05). Scanning electron microscopy showed that the pores of the composite gel increased with the increase of Sr2+ concentration. Microscopy and FDA/Pi staining showed that the cells were scattered in the initial stage of cell inoculation. CONCLUSIONS: The composite gel formed by mixing equal proportion of strontium ions may be used as a new type of excellent cell scaffold material in bone tissue engineering.

Key words: Alginate, Strontium, Hydrogel, Pre-osteoblasts, Tissue engineering

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