组织工程修复区早期牙移动压力侧牙周组织的改建

doi:10.19439/j.sjos.2018.05.003

上海口腔医学 ›› 2018, Vol. 27 ›› Issue (5): 461-466.doi: 10.19439/j.sjos.2018.05.003

组织工程修复区早期牙移动压力侧牙周组织的改建

李一涵¹, 张飞飞¹, 包世婕¹, 魏斌^2,*, 宫耀^3,*

1.上海交通大学医学院附属第九人民医院·口腔医学院口腔修复科,2.口腔第一门诊,3.口腔正畸科,国家口腔疾病临床研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海 200011

收稿日期:2018-04-12 出版日期:2018-10-25 发布日期:2018-11-05
通讯作者: 宫耀,E-mail: gongy0328@126.com;魏斌,E-mail：weibin0328@hotmail.com。
作者简介:李一涵（1992-）,男,硕士研究生,E-mail：kqxfjrh123@126.com
基金资助:
上海市科学技术委员会科研计划项目基金（16140902202）; 上海申康医院发展中心临床科技创新项目基金（SHDC2015625）

Study on periodontal responses on the compression side during early tooth movement into alveolar defect regenerated by a tissue engineering bone

LI Yi-han¹, ZHANG Fei-fei¹, BAO Shi-jie¹, WEI Bin², GONG Yao³

1.Department of Prosthodontics, 2.Special Dental Consultation Clinic, 3.Department of Orthodontics, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center of Stomatology, Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology. Shanghai 200011, China

Received:2018-04-12 Online:2018-10-25 Published:2018-11-05

摘要/Abstract

摘要： 目的: 探讨牙槽骨组织工程修复区内早期牙移动时压力侧的牙周改建情况,为组织工程技术在正畸中应用的安全性和可行性提供实验依据。方法: 选取30只新西兰大白兔,通过拔除下颌第一磨牙并扩大拔牙窝,建立双侧牙槽骨的超临界骨缺损,分别以实验组骨髓间充质干细胞（bone marrow mesenchymal stem cells,BMSCs）与颗粒型多孔β磷酸三钙（beta-tricalcium phosphate,β-TCP）支架复合构成的组织工程骨和对照组单纯β-TCP支架进行右侧和左侧骨缺损修复。术后8周,选取6只兔进行植骨区取材,评价2组的成骨效果。对剩余兔进行下颌两侧第二磨牙加力,近中向牵引4周。分别在加力后的第1、2、3、4周各处死6只动物,测量牙移动距离并制作组织学切片,通过H-E染色观察牙周组织变化,抗酒石酸酸性磷酸酶染色法计数压力侧破骨细胞数目。采用SPSS 19.0软件包对数据进行统计学分析。结果: 植骨术后8周,实验组成骨效果优于对照组。牵引4周后,正畸牙在实验组牙槽骨修复区内的移动量大于对照组。牵引第2、3、4周时,实验组移动牙压力侧的破骨细胞数量均高于对照组,差异具有统计学意义。结论: BMSCs复合β-TCP支架能够良好地修复牙槽骨缺损,邻牙在组织工程修复区内早期移动时,再生的牙周组织改建活跃,有加速牙移动的趋势。

关键词: 骨髓间充质干细胞, 颗粒型多孔β磷酸三钙支架, 骨改建, 组织工程, 牙移动, 兔

Abstract: PURPOSE: To explore periodontal responses on the compression side during early tooth movement into alveolar defect regenerated by bone marrow mesenchymal stem cells (BMSCs) and porous granulated beta-tricalcium phosphate（β-TCP） scaffolds. METHODS: Thirty New Zealand rabbits were used to establish bilateral mandibular defective alveolar bone model by extracting the mandibular first molars and expanding the sockets. The right mandibular alveolar defects were filled with a construct of β-TCP scaffolds combined with BMSCs as experimental group. The left alveolar defects were repaired by β-TCP scaffolds alone as control group. Eight weeks later, 6 rabbits were sacrificed to evaluate osteogenesis effect. The other rabbits were loaded orthodontic force to move the bilateral second molars forward for 4 weeks. Six rabbits in each group were sacrificed at 1, 2, 3, and 4 week after orthodontic tooth movement (OTM). The distance of OTM was measured, and the status of periodontal tissues was observed by H-E staining. The number of osteoclasts on the compression side of tooth was counted by tartrate-resistant acid phosphatase histochemistry. The results were compared between groups using SPSS 19.0 software package. RESULTS: After 8 weeks of bone grafting, the osteogenesis effect of the experimental group was better than the control group. The OTM distance in the experimental area was higher than that in the control area. At 2, 3 and 4 week of OTM, the number of osteoclasts in the experimental group was significantly higher than that in the control group. CONCLUSIONS: A tissue-engineered complex with β-TCP scaffolds and BMSCs could well repair the alveolar bone defect. When the adjacent tooth moved into regenerated area, the new periodontal tissue had an active response, promoting to accelerate tooth movement.

Key words: Bone mesenchymal stem cells, Porous granulated beta-tricalcium phosphate scaffolds, Bone remodeling, Tissue engineering, Tooth movement, Rabbit

中图分类号:

R783.5

李一涵, 张飞飞, 包世婕, 魏斌, 宫耀. 组织工程修复区早期牙移动压力侧牙周组织的改建[J]. 上海口腔医学, 2018, 27(5): 461-466.

LI Yi-han, ZHANG Fei-fei, BAO Shi-jie, WEI Bin, GONG Yao. Study on periodontal responses on the compression side during early tooth movement into alveolar defect regenerated by a tissue engineering bone[J]. Shanghai Journal of Stomatology, 2018, 27(5): 461-466.

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组织工程修复区早期牙移动压力侧牙周组织的改建

Study on periodontal responses on the compression side during early tooth movement into alveolar defect regenerated by a tissue engineering bone

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