上海口腔医学

上海口腔医学 ›› 2017, Vol. 26 ›› Issue (5): 465-470.doi: 10.19439/j.sjos.2017.05.001

• 论著 • 上一篇    下一篇

淫羊藿苷对大鼠正畸牙移动过程中牙槽骨改建的影响

杨盼盼1, 2, 王旭霞2, 3, 陈云4, 汪倩倩1, 2, 王英姿1, 2, 张君1, 2   

  1. 1.山东大学口腔医学院 正畸科,山东 济南 250012；
    2.山东省口腔生物医学重点实验室,山东 济南 250012；
    3.山东大学口腔医学院 口腔颌面外科,山东 济南 250012；
    4.南京医科大学附属口腔医院 儿童牙病科,江苏 南京 210029
  • 收稿日期:2017-03-30 修回日期:2017-05-04 出版日期:2017-10-25 发布日期:2017-11-23
  • 通讯作者: 张君,E-mail：zhangj@sdu.edu.cn
  • 作者简介:杨盼盼（1991-）,女,硕士, E-mail:c8yangpanpan@163.com
  • 基金资助:
    国家自然科学基金 (81371180); 山东省科技发展计划课题（2014GGH218024）

Effect of icraiin on turnover of alveolar bone during orthodontic tooth movement in rats

YANG Pan-pan1, 2, WANG Xu-xia2, 3, CHEN Yun4, WANG Qian-qian1, 2, WANG Ying-zi1, 2, ZHANG Jun1, 2   

  1. 1.Department of Orthodontics,School of Stomatology, Shandong University. Jinan 250012, Shandong Province;
    2.Shandong Provincial Key Laboratory of Oral Biomedicine. Jinan 250012, Shandong Province;
    3.Department of Oral and Maxillofacial Surgery, School of Stomatology, Shandong University. Jinan 250012, Shandong Province;
    4.Department of Pediatric, College of Stomatology, Nanjing Medical University. Nanjing 210029, Jiangsu Province, China
  • Received:2017-03-30 Revised:2017-05-04 Online:2017-10-25 Published:2017-11-23

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摘要： 目的研究淫羊藿苷对大鼠正畸牙移动过程中牙槽骨改建的作用机制。方法48只雄性Sprague-Dawley（SD）大鼠,建立正畸牙移动模型,随机分为对照组和淫羊藿苷组,每组分为4个亚组,每个亚组6只。实验组以灌胃方式给予大鼠淫羊藿苷20 mg/（kg·d）,对照组给予等体积溶剂。各组大鼠在7、14、21、28 d处死,分离出上颌骨作为标本,测量上颌第一磨牙近中移动距离。所有标本做脱钙处理,制成上颌牙周组织切片,分别采用H-E染色、抗酒石酸酸性磷酸酶（tartrate resistant acid phosphatase,TRAP）染色观察移动牙周围牙周膜及牙槽骨的改建情况。采用SPSS16.0软件包对数据进行统计学分析。结果淫羊藿苷组牙移动距离显著大于对照组（P<0.05）。光镜下显示,淫羊藿苷组压力侧破骨细胞数目在第7 天时达到高峰,显著高于对照组;在第14天时与对照组持平,至28 d时低于对照组。结论淫羊藿苷通过促进牙槽骨的改建而加速正畸牙移动。

关键词: 淫羊藿苷, 破骨细胞, 正畸牙移动, 骨改建

Abstract: PURPOSE: To observe the effect of icariin on turnover of paradentium as well as the mechanism of effects on osteoclast during orthodontic tooth movement in rats. METHODS: Forty-right SD rats were used to establish animal models of OTM and divided into two groups randomly: the control group and the experimental group. Rats in the experimental group were given 20 mg/（kg·d） icariin by intragastric administration, while rats in the control group received solvent with the same volume. The rats were sacrificed in batch on the 7th, 14th, 21st and 28th day after orthodontic treatment. The amount of tooth movement was measured, and histomorphometric analysis based on slices from periodontium adjacent to the maxillary first molars was used to observe new bone formation, bone resorption and quantify osteoclasts. Statistical analysis was performed by using SPSS 16.0 software package. RESULTS: The movement distance of the first molar was significantly larger in the icariin group than that in the control group (P<0.05). In the icariin group, the number of osteoclasts was significantly higher than the control group at the 7th day and then demonstrated a steady decrease. CONCLUSIONS: Icariin could accelerate OTM in rats through promoting bone turnover of alveolar bone.

Key words: Icariin, Osteoclast, Orthodontic tooth movement, Bone turnover

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